mrc 5 human fetal lung fibroblast cells Search Results


90
Binax Inc mrc-5 (human fetal lung fibroblast cell line
Mrc 5 (Human Fetal Lung Fibroblast Cell Line, supplied by Binax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mrc-5 (human fetal lung fibroblast cell line/product/Binax Inc
Average 90 stars, based on 1 article reviews
mrc-5 (human fetal lung fibroblast cell line - by Bioz Stars, 2026-02
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90
JCRB Cell Bank human normal lung fibroblasts mrc-5
Human Normal Lung Fibroblasts Mrc 5, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human normal lung fibroblasts mrc-5/product/JCRB Cell Bank
Average 90 stars, based on 1 article reviews
human normal lung fibroblasts mrc-5 - by Bioz Stars, 2026-02
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90
Tibotec Pharmaceuticals human fetal lung-derived normal fibroblast mrc-5 cells
Human Fetal Lung Derived Normal Fibroblast Mrc 5 Cells, supplied by Tibotec Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human fetal lung-derived normal fibroblast mrc-5 cells/product/Tibotec Pharmaceuticals
Average 90 stars, based on 1 article reviews
human fetal lung-derived normal fibroblast mrc-5 cells - by Bioz Stars, 2026-02
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90
KAC Co Ltd normal human fetal pulmonary fibroblast cell line mrc-5
Expression of the vitamin D receptor and vitamin D-metabolizing enzymes in human <t>fibroblasts,</t> and VD 3 -dependent suppression of the induction of IL-1β gene expression in pulmonary fibroblasts by bleomycin. (A) To assess gene expression in MRC-5 and MRC-5 SV1 TG1 cells, RT-PCR was performed using specific primers for the vitamin D receptor (VDR) or vitamin D-metabolizing enzymes, including CYP27A1 (27A1), CYP2R1 (2R1), and CYP27B1 (27B1). (B) MRC-5 SV1 TG1 cells were treated with 25 µg/ml bleomycin for 48 h and RT-PCR was performed using specific primers for αSMA, IL-1β, and GAPDH. (C, D) Band intensities of the PCR products were converted to numerical data by image analysis software, and the data for αSMA and IL-1β were normalized by the GAPDH value.
Normal Human Fetal Pulmonary Fibroblast Cell Line Mrc 5, supplied by KAC Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/normal human fetal pulmonary fibroblast cell line mrc-5/product/KAC Co Ltd
Average 90 stars, based on 1 article reviews
normal human fetal pulmonary fibroblast cell line mrc-5 - by Bioz Stars, 2026-02
90/100 stars
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90
Obio Technology Corp Ltd human fetal lung fibroblast cell mrc-5
Expression of the vitamin D receptor and vitamin D-metabolizing enzymes in human <t>fibroblasts,</t> and VD 3 -dependent suppression of the induction of IL-1β gene expression in pulmonary fibroblasts by bleomycin. (A) To assess gene expression in MRC-5 and MRC-5 SV1 TG1 cells, RT-PCR was performed using specific primers for the vitamin D receptor (VDR) or vitamin D-metabolizing enzymes, including CYP27A1 (27A1), CYP2R1 (2R1), and CYP27B1 (27B1). (B) MRC-5 SV1 TG1 cells were treated with 25 µg/ml bleomycin for 48 h and RT-PCR was performed using specific primers for αSMA, IL-1β, and GAPDH. (C, D) Band intensities of the PCR products were converted to numerical data by image analysis software, and the data for αSMA and IL-1β were normalized by the GAPDH value.
Human Fetal Lung Fibroblast Cell Mrc 5, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human fetal lung fibroblast cell mrc-5/product/Obio Technology Corp Ltd
Average 90 stars, based on 1 article reviews
human fetal lung fibroblast cell mrc-5 - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Expression of the vitamin D receptor and vitamin D-metabolizing enzymes in human fibroblasts, and VD 3 -dependent suppression of the induction of IL-1β gene expression in pulmonary fibroblasts by bleomycin. (A) To assess gene expression in MRC-5 and MRC-5 SV1 TG1 cells, RT-PCR was performed using specific primers for the vitamin D receptor (VDR) or vitamin D-metabolizing enzymes, including CYP27A1 (27A1), CYP2R1 (2R1), and CYP27B1 (27B1). (B) MRC-5 SV1 TG1 cells were treated with 25 µg/ml bleomycin for 48 h and RT-PCR was performed using specific primers for αSMA, IL-1β, and GAPDH. (C, D) Band intensities of the PCR products were converted to numerical data by image analysis software, and the data for αSMA and IL-1β were normalized by the GAPDH value.

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Pulmonary activation of vitamin D 3 and preventive effect against interstitial pneumonia

doi: 10.3164/jcbn.19-48

Figure Lengend Snippet: Expression of the vitamin D receptor and vitamin D-metabolizing enzymes in human fibroblasts, and VD 3 -dependent suppression of the induction of IL-1β gene expression in pulmonary fibroblasts by bleomycin. (A) To assess gene expression in MRC-5 and MRC-5 SV1 TG1 cells, RT-PCR was performed using specific primers for the vitamin D receptor (VDR) or vitamin D-metabolizing enzymes, including CYP27A1 (27A1), CYP2R1 (2R1), and CYP27B1 (27B1). (B) MRC-5 SV1 TG1 cells were treated with 25 µg/ml bleomycin for 48 h and RT-PCR was performed using specific primers for αSMA, IL-1β, and GAPDH. (C, D) Band intensities of the PCR products were converted to numerical data by image analysis software, and the data for αSMA and IL-1β were normalized by the GAPDH value.

Article Snippet: A normal human fetal pulmonary fibroblast cell line (MRC-5) and an immortalized cell line derived from MRC-5 (MRC-5 SV1 TG1) (KAC Co., Ltd., Kyoto, Japan) were maintained in α-minimal essential medium (α-MEM) containing 10% fetal calf serum (FCS) and antibiotics (Life Technologies Japan Ltd., Tokyo, Japan) at 37°C under 5% CO 2 .

Techniques: Expressing, Gene Expression, Reverse Transcription Polymerase Chain Reaction, Software

Pretreatment of pulmonary fibroblasts with VD 3 suppresses bleomycin-induced expression of pro-fibrotic genes, fibrosis markers, and inflammatory markers. (A) MRC-5 SV1 TG1 cells underwent pretreatment with VD 3 or 1,25(OH) 2 D 3 overnight and then were incubated with 25 µg/ml bleomycin for 24 h, after which RT-PCR was performed using specific primers for αSMA, COLA2, SPP1, IL-1β, TGF-β1, and β-actin (ACTB). (B–D) Band intensities of the PCR products were converted to numerical data by image analysis software, and the data for SPP1, IL-1β, and TGF-β1 were normalized by the β-actin value.

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Pulmonary activation of vitamin D 3 and preventive effect against interstitial pneumonia

doi: 10.3164/jcbn.19-48

Figure Lengend Snippet: Pretreatment of pulmonary fibroblasts with VD 3 suppresses bleomycin-induced expression of pro-fibrotic genes, fibrosis markers, and inflammatory markers. (A) MRC-5 SV1 TG1 cells underwent pretreatment with VD 3 or 1,25(OH) 2 D 3 overnight and then were incubated with 25 µg/ml bleomycin for 24 h, after which RT-PCR was performed using specific primers for αSMA, COLA2, SPP1, IL-1β, TGF-β1, and β-actin (ACTB). (B–D) Band intensities of the PCR products were converted to numerical data by image analysis software, and the data for SPP1, IL-1β, and TGF-β1 were normalized by the β-actin value.

Article Snippet: A normal human fetal pulmonary fibroblast cell line (MRC-5) and an immortalized cell line derived from MRC-5 (MRC-5 SV1 TG1) (KAC Co., Ltd., Kyoto, Japan) were maintained in α-minimal essential medium (α-MEM) containing 10% fetal calf serum (FCS) and antibiotics (Life Technologies Japan Ltd., Tokyo, Japan) at 37°C under 5% CO 2 .

Techniques: Expressing, Incubation, Reverse Transcription Polymerase Chain Reaction, Software